differential proteomics analysis of proteins from human diabetic and age-related cataractous lenses
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چکیده
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منابع مشابه
Proteomic analysis of water insoluble proteins from normal and cataractous human lenses.
PURPOSE The purpose of the study was to compare and analyze the composition of crystallin species that exist in the water insoluble-urea soluble (WI-US) and water insoluble-urea insoluble (WI-UI) protein fractions of a human cataractous lens and an age-matched normal lens. METHODS The water soluble (WS) and water insoluble (WI) protein fractions from a 68-year-old normal lens and a 61-year-ol...
متن کاملPhosphoproteomics characterization of novel phosphorylated sites of lens proteins from normal and cataractous human eye lenses
PURPOSE Post-translational modification (PTM) of lens proteins is believed to play various roles in age-related lens function and development. Among the different types of PTM, phosphorylation is most noteworthy to play a major role in the regulation of various biosignaling pathways in relation to metabolic processes and cellular functions. The present study reported the quantitative analysis o...
متن کاملStudies on protein and taurine in normal, senile and diabetic cataractous human lenses.
An attempt has been made to explore the possible role of Taurine in cataractogenesis. Normal lenses were obtained from eye bank donors and cataractous lenses from patients who had undergone surgery for cataract extraction. Lenses were weighed and homogenised. Extraction, isolation and estimation of protein and taurine were carried out. It has been found that the lens wet weight increased progre...
متن کاملDiscrepant expression of microRNAs in transparent and cataractous human lenses.
PURPOSE Age-related cataracts are considered to be a pathological condition that arise as senescence progresses. However, little is known about the function of microRNAs (miRNAs) in the formation of age-related cataracts. The purpose of this study was to identify possible differences in miRNA expression in the central epithelium of transparent and age-related cataractous human lenses. METHODS...
متن کاملFree and bound water in normal and cataractous human lenses.
PURPOSE To analyze free and total water in human normal and cataractous lenses. METHODS Thermogravimetric analysis was used to determine total water, and differential scanning calorimetry was used for free water. RESULTS In normal human lenses, the total water content of the nucleus remained unchanged with age, but the state of the water altered. The ratio of free to bound water increased s...
متن کاملAnalysis of nuclear fiber cell compaction in transparent and cataractous diabetic human lenses by scanning electron microscopy
BACKGROUND Compaction of human ocular lens fiber cells as a function of both aging and cataractogenesis has been demonstrated previously using scanning electron microscopy. The purpose of this investigation is to quantify morphological differences in the inner nuclear regions of cataractous and non-cataractous human lenses from individuals with diabetes. The hypothesis is that, even in the pres...
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عنوان ژورنال:
journal of research in medical sciencesجلد ۱۸، شماره ۱۱، صفحات ۰-۰
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